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KMID : 0385019870030010051
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Korean Journal of Laboratory Animal Science
1987 Volume.3 No. 1 p.51 ~ p.56
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Phytomitogen Induced Blast Transformation of Korean Native Goat:Ⅰ. Effect of Medium, Phytomitogen and Cell Concentration on the Uptake of 3H-Thymidine
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이주홍/Lee, Ju Hong
김종수/이국천/조희택/Kim, Jong Shu/Lee, Kook Cheol/Cho, Hee Taek
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Abstract
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The present study has been carried out to investigate the optimal condition on the blastogenesis of Korean Native Goat lymphocytes. A microculture system in conjunction with a semiautomatic multiple sample harvester(SAMSH) was used to study the in vivio optimal condition of Korean Native Goat lymphocytes.
Data were presented to show many variables that are involved in studying the responses of Korean Native Goat lymphocyte in a microculture system to the stimulation of phytohemagglutinin (PHA) and lipopolysaccharide( LPS ).
Analysis indicated that the conditions for optimal PHA and LPS as measured by incorporation of ³H-TdR include:
1. Use of RPMI-1640 as culture medium.
2. Use of 0.2㎍ of PHA, 0.4㎍ of LPS, per culture, respectively.
3. Use of 1×10^6, 1×10^7 cells in PHA and LPS per culture, respectively.
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